Homogeneous and Universal Detection of Various Targets with a Dual-Step Transduced Toehold Switch Sensor

Toehold switch sensors represent a class of new advances that allow specific targets to trigger in situ expression of a protein reporter. Although they offer unique advantages of a label-free nature and high portability, they generally require repeated sequence design, high expenditure, and laborious optimization of toehold switch sequences according to different targets. To simplify the sensing process further and to improve its practicability, we innovatively introduce a dual-step pre-transduction upon traditional toehold switch sensor. Through two successive toehold-mediated strand-displacement reactions that are initiated, respectively, by a linear and an associative trigger, different DNAs, RNAs, or ligands of functional nucleic acids can be generally transduced into the input of one high-performance toehold switch sensor. This advance greatly increases the target range. Furthermore, the whole process is signal-on, homogeneous, and free of any requirements for complicated operations such as probe labeling, separation, and reconstruction of the toehold switch, being promising and practical even in portable or point-of-care assays.     

频率域电磁法在采空区探测中的应用

隐伏采空区的探测已成为近年来研究课题,采用无人机搭载发射装置,高空平面布置探测线和测点,克服在陡峭的山坡难以布置探测工作,消除地形地貌及其它条件对探测工作带的误差。对已知空区的探测和分析,验证了该方法的有效性和精确程度,为后续其它探测提供了频率域区间和参数校正。研究表明:此方法具有适用性强、工作效率高和精确度高优点,是一种可用于隐伏空区的探测方法,分析过程中可以辨别采空区变形情况。     

5通道铁磁探测仪的研发与锅炉管堵塞检测

描述了5通道铁磁探测仪的研发和应用，该仪器能在管外发现发电锅炉过热器管和再热器管内是否有铁锈堆积，并评估堵塞程度，目的是通过检测防止爆管事故。采用磁学探测方法，在管外表面的1条圆周线上布置5个测量点，进行了有限元数值模拟和标定曲线的计算。对计算结果进行了实验验证，与计算值相比较，测量值的标准误差（相对误差）为5.2%。现场检测得到的堵塞面积与管内孔的面积之比，与X射线摄影测得的结果比较，确定铁磁探测仪检测的标准误差（绝对误差）为3.9%。通过为5家热电厂进行过热器管和再热器管堵塞检测，发现超标堵塞30多处，解剖验证无一误报，所以用该仪器进行堵塞检测能够防止上述锅炉管爆管事故。     

石墨烯复合功能材料及其在重金属离子检测的电化学研究与应用

石墨烯(GR)是典型的单原子碳纳米材料,具有独特的二维共辄平面结构,其高活性的比表面积和突出的导电性能,在电催化和敏感材料制备领域已得到广泛的应用。氧化石墨烯(GO)作为GR的前驱体,存在大量的含氧官能团,具有良好的水溶分散性。大量GO含氧官能团的介入会破坏其K-7T共辘结构,导致其电学性能变差。GO通过化学、水热合成或直接电化学还原方法可有效修复其共辄平面结构,得到导电性良好的还原氧化石墨烯(rGO),即GR.单组分的GR材料在实际应用中仍存在某些局限性,如电学活性相对较弱,与其它材料加工复合性能较差等。将GR、G O材料与其它功能材料进行复合,可进一步改善复合物的物理或化学性能,如分散性、加工修饰和电催化活性等。综述了石墨烯材料与金属及其氧化物纳米粒子、聚合物、掺杂原子、导电离子液体、碳纳米材料等功能材料复合后,能形成可调控的微结构,具有改性的化学性质和协同发挥的电学效应,表现出显著的电子传递能力及其功能性作用。论述了GR功能化修饰的复合材料作为敏感界面,构筑基于重金属离子检测的电化学生物传感器,可以实现对Pb2+,Hg2+,C『+等多种重金属离子的同时或分别检出,提出了GR复合制备材料的纳米结构特征、功能修饰作用对于提高传感器的电催化活性和选择性性能等方面的应用,并对该研究领域进行了总结与展望。     

Gait event detection algorithm based on smart insoles

Gait analysis is an effective clinical tool across a wide range of applications. Recently, inertial measurement units have been extensively utilized for gait analysis. Effective gait analyses require good estimates of heel‐strike and toe‐off events. Previous studies have focused on the effective device position and type of triaxis direction to detect gait events. This study proposes an effective heel‐strike and toe‐off detection algorithm using a smart insole with inertial measurement units. This method detects heel‐strike and toe‐off events through a time‐frequency analysis by limiting the range. To assess its performance, gait data for seven healthy male subjects during walking and running were acquired. The proposed heel‐strike and toe‐off detection algorithm yielded the largest error of 0.03 seconds for running toe‐off events, and an average of 0–0.01 seconds for other gait tests. Novel gait analyses could be conducted without suffering from space limitations because gait parameters such as the cadence, stance phase time, swing phase time, single‐support time, and double‐support time can all be estimated using the proposed heel‐strike and toe‐off detection algorithm.     

A signal adaptive diffusion filter for video coding: Mathematical framework and complexity reductions

In this paper we combine video compression and modern image processing methods. We construct novel iterative filter methods for prediction signals based on Partial Differential Equation (PDE) based methods. The mathematical framework of the employed diffusion filter class is given and some desirable properties are stated. In particular, two types of diffusion filters are constructed: a uniform diffusion filter using a fixed filter mask and a signal adaptive diffusion filter that incorporates the structures of the underlying prediction signal. The latter has the advantage of not attenuating existing edges while the uniform filter is less complex. The filters are embedded into a software based on HEVC with additional QTBT (Quadtree plus Binary Tree) and MTT (Multi-Type-Tree) block structure. In this setting, several measures to reduce the coding complexity of the tool are introduced, discussed and tested thoroughly. The coding complexity is reduced by up to 70% while maintaining over 80% of the gain. Overall, the diffusion filter method achieves average bitrate savings of 2.27% for Random Access having an average encoder runtime complexity of 119% and 117% decoder runtime complexity. For individual test sequences, results of 7.36% for Random Access are accomplished.     

A Colorimetric Artificial Olfactory System for Airborne Improvised Explosive Identification

The detection of ultralow or nonvolatile target analytes remains a significant challenge for artificial olfactory systems even after decades of development, which severely limits their widespread application. To overcome this challenge, an artificial olfactory system based on a colorimetric hydrogel array is constructed for the first time as a universal representative. As an effective extension of conventional artificial olfactory systems that integrates the merits of its predecessors, the proposed system accurately mimics olfactory mucosa and specific odorant binding proteins using hydrogels endowed with specific colorimetric reagents for the detection of hypochlorite, chlorate, perchlorate, urea, and nitrate. Therefore, the proposed system is capable of detecting and discriminating between these five airborne improvised explosive microparticulates with a detection limit as low as 39.4 pg. Additionally, the system demonstrates good reusability over ten cycles, rapid response time of ≈0.2 s, and excellent discrimination properties, despite significant variation. This proof-of-concept study on colorimetric artificial olfactory systems yields a novel strategy for the direct and discriminative detection of nonvolatile airborne microparticulates.     

关于硬件木马电路检测技术分析及发展趋势探讨

本文结合HTH电路的复杂性等特征分析与实际应用现状,研究了HTH电路的具体检测技术方法并对当前热点技术进行对比分析,探讨了HTH电路检测方法的技术发展趋势。     

基于序列模型的无线传感网入侵检测系统

随着物联网(IoT)的快速发展，越来越多的IoT节点设备被部署，但伴随而来的安全问题也不可忽视。IoT的网络层节点设备主要通过无线传感网进行通信，其相较于互联网更开放也更容易受到拒绝服务等网络攻击。针对无线传感网面临的网络层安全问题，提出了一种基于序列模型的网络入侵检测系统，对网络层入侵进行检测和报警，具有较高的识别率以及较低的误报率。另外，针对无线传感网节点设备面临的节点主机设备的安全问题，在考虑节点开销的基础上，提出了一种基于简单序列模型的主机入侵检测系统。实验结果表明，针对无线传感网的网络层以及主机层的两个入侵检测系统的准确率都达到了99%以上，误报率在1%左右，达到了工业需求，这两个系统可以全面有效地保护无线传感网安全。